Proteomics and Protein-Protein Interactions

The Alam laboratory is using several biophysical approaches to study protein-protein interactions including Biacore assays, which involves Surface Plasmon Resonance (SPR) measurements to study binding kinetics, affinity and energetics. His group is working to develop highly sensitive assays for detection and characterization of antigen specific T and B cell responses. They are also utilizing state-of-the-art techniques in protein fractionation (2D-gel, Nano-LC) and Proteomics applications involving mass spectrometry for biomarker discovery. Dr Alam’s laboratory also is also using a Laser Capture Microdissection (LCM) system to identify and microdissect immune cells of interest for down-stream applications in proteomics and mRNA analysis.  The DHVI Proteomics Core has three BiaCore units, a laser dissection microscope, and an isothermal calorimiter. Taken together, the Proteomics Core facility brings unique strengths and ability to characterize vaccine immunogens for the DHVI vaccine development teams.